Journal: Regenerative Therapy
Article Title: Mesenchymal stem cells reverse thymus aging by reprogramming the DNA methylation of thymic epithelial cells
doi: 10.1016/j.reth.2024.03.008
Figure Lengend Snippet: MSCs induce NGF demethylation to regulate the expression of KRT17 and FOXJ1. A MSCs reverse the methylation of transcription factors and the regulatory network of target genes during TEC aging (orange triangles represent transcription factors, and blue circles represent target genes). B The transcription factor NGF was associated with the RNA-seq expression of the target genes KRT17 and FOXJ1 during the reversal of TEC senescence by MSCs. The horizontal coordinate represents the expression of the transcription factor NGF, and the ordinate represents the expression of the target gene. C The mRNA expression levels of demethylated NGF, KRT17 and FOXJ1 were analyzed via qPCR. D The expression of NGF was detected by immunofluorescence (400×). E The expression of the TEC proliferation marker Ki67 was detected by immunofluorescence (400×). F Regulatory role of decreased methylation of the NGF/KRT17/FOXJ1 axis in the MSC-mediated reversal of TEC senescence. The measured values represent the mean ± standard deviation (`X ± s) of three replicate experiments. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).
Article Snippet: Anti-mouse anti-human antibodies against Ki67 and NGF (1R1000 ∼ (th) ServiceBio) were added for staining.
Techniques: Expressing, Methylation, RNA Sequencing, Immunofluorescence, Marker, Standard Deviation